GE Cytiva Superose 6 Increase 5/150 GL FPLC Column

Superose 6 Increase 5/150 GL

29091597

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Overview

Superose™ 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range Mr~ 5K to ~ 5M.

Wide fractionation range: optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments

Very high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity

High flow rates: rigid beads give excellent pressure/flow properties

Enhanced performance: improved resolution and runtime compared to predecessor

“Increase”: Cytiva's new generation of size exclusion chromatography columns

Superose™ 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose™ 6 Increase columns deliver higher resolution protein purification in shorter runtimes.

Fractionation of large proteins and protein complexes

Suitable for both preparative and analytical applications, Superose™ 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.

Superose™ 6 and Superdex™ 200 analysis ranges are complementary

Superose™ 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding Mr ~400 000. The optimal fractionation range of Superdex™ 200 Increase is complementary, providing higher resolution for proteins with molecular weights below Mr ~400 000.

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.

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