{"product_id":"millicell-hy-multilayer-cell-culture-flask-t-600-sterile-vent-cap-new-case-3d","title":"Millicell HY Multilayer Cell Culture Flask T-600 Sterile Vent Cap New Case 3D","description":"\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eNew complete case of 16 flasks \u003c\/p\u003e\n\u003cbr\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eGeneral description\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eThe Millicell® HY Flask is designed to enhance cell culture efficiency across various research applications, including cell banking, cell-based assays, and virus production.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eFeatures and Benefits\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eMultilayer Design: Available in 3- and 5-layer configurations, providing a larger surface area for cell growth while maintaining a compact footprint similar to traditional T-flasks\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eEnhanced Accessibility: Designed for better pipette access, accommodating larger volume pipettes (up to 25 mL)\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eIncreased Efficiency: Cultivate more cells in the same space, maximizing research output\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eSpace Optimization: Achieves significant space savings compared to other multilayer flasks on the market\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003ePreparation Note\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eTo seed cells, fill the Millicell HY T-600 Flask with the desired growth media.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eAdd desired number of cells to achieve typical seeding density.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eMix the cells and media by raising the end of the flask and pipetting the solution up and down several times until a homogeneous suspension is achieved. Note: Failure to adequately mix cells into a homogeneous suspension may result in an uneven distribution of cells across the layers of the Millicell HY Flask.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eBriefly lay the flask on its side to equilibrate the liquid volume equally among the layers of the flask.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eTilt the flask on its end to partition the liquid volume for each layer of the flask.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eLay the flask down to spread the cell suspension evenly across the entire surface of each layer.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eDepending on the volume it may be necessary to rock the flask on all four corners to ensure that the entire surface wets. Note: If the liquid volume on any of the layers changes during the wetting procedure (e.g., spilling of liquid from top layer to lower layers), repeat steps 4–6 to ensure even distribution of liquid among all layers.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eReturn the flask to a vertical position when transporting to the incubator. Lay the flask flat in the incubator (as in step 6) and culture cells under appropriate conditions.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eIf media exchange is required, either aspirate or pour the media from the Millicell HY T-600 Flask. Note: Due to the larger surface area of the Millicell Flasks, it may be necessary to let the flask stand on its side for several minutes following each aspiration so that all residual liquid pools to a common point, allowing for a second aspiration to completely remove the remaining liquid.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eAdd appropriate volume of fresh media, and repeat steps 4–8.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eTo harvest cells, remove media as in step 9.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eAdd desired amount of appropriate wash solution (e.g., PBS or 0.02% EDTA) to the flask and repeat steps 4–7.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eRemove wash solution as in step 9.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eAdd desired amount of dissociation enzyme (e.g., 0.25% trypsin\/EDTA or equivalent) according to preferred protocol, repeat steps 4–8, and incubate. Volume of dissociation enzyme required per cm2 does not need to be altered from T-flask protocol. Note: Ensure that all cells have completely dissociated from the surface of the flask via observation under microscope. Failure to allow complete dissociation of cells from culture surface (such that all cells are freely floating) may result in reduced cell yields.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eIf using trypsin, add desired volume of inactivating solution, such as serum-containing media.\u003c\/p\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eCollect cells either by aspirating or pouring, as in step 9.\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp dir=\"ltr\" style=\"margin-top:0; margin-bottom:0;\"\u003eShelf SRm\u003c\/p\u003e","brand":"Biotek2020 We Make Science Easier","offers":[{"title":"Default Title","offer_id":52743626948919,"sku":"EB-267145157474","price":80.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0927\/1302\/5847\/files\/57_900ef2ab-42b0-4856-9277-a7cdef690404.jpg?v=1785483111","url":"https:\/\/biotek2020.com\/products\/millicell-hy-multilayer-cell-culture-flask-t-600-sterile-vent-cap-new-case-3d","provider":"Biotek2020 We Make Science Easier","version":"1.0","type":"link"}